Acid Protease
Bulk Enzyme for Brewing, Distilling, Feed & Protein Hydrolysis
Acid protease from Aspergillus niger offers dependable proteolysis for acidic industrial systems. Available in 25 kg drums, activity 50,000–100,000 U/g, optimal pH 2.0–5.0, 30–50 °C. Ships globally to 80+ countries. Request a free 100 g trial with COA to validate in your process before bulk order.
Years experience
Countries shipped
ISO
Acid Protease Enzyme Powder
50,000 – 100,000 U/g
Applications
Acid protease hydrolyses proteins into peptides and amino acids under acidic conditions, supporting commercial processes where low pH, controlled temperature, and repeatable batch performance are critical.
Alcohol & Spirits
Releases amino nitrogen from grain proteins during acidic fermentation at pH 3.5–4.5. Typical dose 0.03–0.08 % of grist. Supports consistent performance in baijiu, whisky, and fuel ethanol mashes.
Learn more →Brewing
Hydrolyses high-MW proteins that contribute to chill haze. Helps improve wort clarity and foam stability in high-adjunct beer production. Typical dose 20–50 g/hL at mash.
Learn more →Animal Feed
Performs in the acid crop and proventriculus at pH 2–4, supporting protein digestibility and feed conversion efficiency. Typical inclusion 50–150 g/ton in premixes.
Protein Hydrolysis
Produces peptides and free amino acids from soy, pea, whey, and collagen at pH 3.0–4.5 and 45 °C for controlled hydrolysate manufacturing.
Soy Sauce & Seasonings
Accelerates moromi-stage protein breakdown in koji-based soy sauce and miso production. Supports umami development and can shorten maturation timelines in controlled fermentation lines.
Leather Processing
Supports bating and dehairing at acidic pH, helping reduce reliance on harsher chemical treatments. Suitable for cleaner grain surface control and lower effluent burden.
Product Specifications
| Enzyme Name | Acid Protease (EC 3.4.23.18) |
| Activity | 50,000 – 100,000 U/g |
| Optimal pH | 2.0 – 5.0 |
| Optimal Temperature | 30°C – 50°C |
| Appearance | Light yellow to brown powder |
| Source | Aspergillus niger |
| Shelf Life | 12 months (sealed, cool, dry place) |
| Packaging | 25 kg fiber drums / custom packaging |
| MOQ | 25 kg |
| Certifications | ISO 9001, HALAL, KOSHER, Food Grade |
Why Source From Us
Low-pH stability
Aspergillus niger–sourced acid protease remains active across pH 2.0–5.0, delivering reliable performance in acidic fermentations and other low-pH workflows.
Complete documentation
Each batch ships with COA, TDS, ISO 9001, HALAL, KOSHER, and Food Grade statements to simplify QA and export compliance in 80+ markets.
Tailored activity grades
Select 50,000 U/g for feed or 100,000 U/g for concentrated hydrolysis, brewing, distilling, and condiment processes requiring precise dosing.
Small MOQ & global supply
MOQ from 25 kg with 12-month shelf life and worldwide logistics. Trial quantities available for validation before full-scale procurement.
Frequently Asked Questions
What is acid protease and what does it do?
Acid protease is a low-pH endoprotease that hydrolyses peptide bonds, converting proteins into peptides and amino acids. Industrial users apply it in acidic mashes, fermentations, feed lines, and hydrolysis plants.
What is the EC number of acid protease?
EC 3.4.23.18. Classified as an aspartic-type endoprotease with acidic process suitability.
What's the difference between acid protease, neutral protease, and alkaline protease?
Acid protease works at pH 2–5, neutral protease near pH 6–7, alkaline protease above pH 8. Selection depends on substrate, process pH, and performance needs.
Is acid protease the same as aspartic acid protease?
Yes, both terms describe enzymes with a catalytic mechanism involving two aspartate residues for stable activity in acidic environments.
How much acid protease do I dose in a baijiu or whisky mash?
Typical dose is 0.03–0.08 % of grist. Adjust based on grain bill, solids, pH, and temperature. Share process data for trial recommendations.
Is your acid protease food grade, HALAL, and KOSHER?
Yes, supplied with Food Grade, HALAL, and KOSHER documentation plus COA and TDS for QA and procurement.
What's the optimal pH and temperature for acid protease?
Optimal pH 2.0–5.0 and 30–50 °C, matching acidic fermentation, low-pH mashing, and controlled hydrolysis conditions.
How do I request a free sample and COA?
Complete the contact form with application, substrate, pH, and batch size. We will send a free 100 g sample with COA and quote bulk supply within 24 h.
Get a Free Sample & Quote
Provide substrate type, process pH and temperature, and desired hydrolysis outcome. We’ll recommend grade (50k or 100k U/g), ship a 100 g trial with COA, and quote for 25 kg drums within 24 h.